| 1. | Construction of recombinant retroviral vectors expressing b7 - 1 gene in cell 1基因的重组逆转录病毒载体构建及真核表达 |
| 2. | Construction and expression of retroviral vector containing bone morphogenetic protein - 7 gene 7基因逆转录病毒载体的构建及其表达 |
| 3. | Tumor necrosis factor receptor position196 polymorphism in systemic lupus erythematosus and establishment of recombinant retroviral vector 196位点基因多态性及重组反转录病毒载体的构建 |
| 4. | Xu y , tian yk , tian xb , et al . construction and identification of recombinant retroviral vector with controllable human preproenkephalin gene j . chinese j anes , 2005 , 25 ( 7 ) : 511 ? 514 徐颖,田玉科,田学愎,等.携带可调控人前脑啡肽原基因逆转录病毒载体的构建及鉴定j .中华麻醉学杂志, 2005 ( 7 ) , 25 : 511 ? 514 |
| 5. | Methods : a retroviral vector containing vegf gene was constructed and it was expressed after it was transferred to bmsc . the protein expression was detected by immuohistochemistry and its effect of stimulating angiogenesis was observed by microvessal count ( mvc ) 方法:构建含vegf基因的逆转录病毒表达载体,通过感染兔bmsc使其获得稳定表达,使用免疫组化的方法检测其蛋白表达,并通过新生血管计数观察其促血管生成作用。 |
| 6. | Results : the retroviral vector containing vegf gene was constructed successfully . immuohistochemistry showed that it expressed vegf protein in bmsc plasma ( brown staining ) and the experimental study confirmed that its mvc was higher than that in control group ( p < 0 . 01 ) . conclusion : gene transfer technology mediated by retroviral vector 结果:成功构建vegf逆转录病毒表达载体,免疫组化方法显示经感染的bmsc胞质中有阳性棕色颗粒出现,且动物实验显示新生血管计数明显高于对照组( p < 0 . 01 ) 。 |